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Registros recuperados : 34 | |
6. | | OBERTI, H.; DALLA RIZZA, M.; REYNO, R.; MURCHIO, S.; ALTIER, N.; ABREO, E. Diversity of Claviceps paspali reveals unknown lineages and unique alkaloid genotypes. Mycologia, 3 March 2020, Volume 112, Issue 2, Pages 230-243. Doi: https://doi.org/10.1080/00275514.2019.1694827 Article history: Received 25 Apr 2019 // Accepted 15 Nov 2019 // Published online: 07 Jan 2020. CONTACT: E. Abreo, eabreo@inia.org.uy. FUNDING: This work was supported by the National Institute of Agricultural Research (INIA) of Uruguay...Biblioteca(s): INIA Tacuarembó. |
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7. | | PERDOMO FERRANDO, E.; MURCHIO, S.; WALASEK, W.; SCHVARTZMAN, C.; MAESO, D.; DALLA RIZZA, M. Evaluación de eventos de tomate con el gen EFR para el control de bacterias patógenas. [p22]. Bloque 2: Mejoramiento por resistencia a enfermedades. In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 4., Jornada Uruguaya de Protección Vegetal, 2., 1° setiembre, 2017, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2017. p. 42. Financiamiento: INIA. Proyecto BT _13.Biblioteca(s): INIA Las Brujas. |
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8. | | DALLA RIZZA, M.; SCHVARTZMAN, C.; MURCHIO, S.; BERRUETA, C.; BOSCHI, F.; LENZI, A.; GIMÉNEZ, G. Field performance of resistant potato genotypes transformed with the EFR receptor from Arabidopsis thaliana in the absence of bacterial wilt (Ralstonia solanacearum). Research article. The Plant Pathology Journal, 2022, vol.38 (3): 239-247. OPEN ACCESS. doi: https://doi.org/10.5423/PPJ.OA.01.2022.0008 Article history: Received 20 January 2022; Revised 9 May 2022; Accepted 10 May 2022. -- Corresponding author: Marco Dalla-Rizza, Email: mdallarizza@inia.org.uy -- Marco Dalla-Rizza and Claudia Schvartzman contributed equally to this...Biblioteca(s): INIA Las Brujas. |
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9. | | OBERTI, H.; SPANGENBERG, G.; COGAN, C.; REYNO, R.; FEIJOO, M.; MURCHIO, S.; DALLA RIZZA, M. Genome-wide analysis of Claviceps paspali: insights into the secretome of the main species causing ergot disease in Paspalum spp. Research article. BMC Genomics, December 2021, Volume 22, Issue 1, Article number 766. Gold Open Access, Green Open Access. doi: https://doi.org/10.1186/s12864-021-08077-0 Article history: Received 24 September 2020; Accepted 11 October 2021; Published 26 October 2021.
Funding: This project was supported by the National Institute of Agricultural Research of Uruguay (INIA) project BT-19, and by project...Biblioteca(s): INIA Las Brujas. |
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10. | | CASTRO, L.; MURCHIO, S.; REYNO, R.; OBERTI, H.; DALLA RIZZA, M. Identificación y análisis de diversidad en colecta de aislados de Claviceps paspali de Uruguay. In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 6., Jornada Uruguaya de Protección Vegetal, 4., 21-22 octubre, 2021, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2021. p. 30. Financiamiento: Proyecto BT-19 INIA.Biblioteca(s): INIA Treinta y Tres. |
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11. | | OBERTI, H.; ABREO, E.; REYNO, R.; FEIJOO, M.; MURCHIO, S.; DALLA RIZZA, M. New draft genome sequence of the ergot disease fungus claviceps paspali. Microbiology Resource Announcements, 16 July 2020, Volume 9, Issue 29, Article number e00498-20. OPEN ACCESS. Doi: https://doi.org/10.1128/MRA.00498-20 Article history: Received 28 May 2020; Accepted 20 June 2020; Published 16 July 2020.
Editor: Antonis Rokas - Vanderbilt University.
Corresponding author: Dalla-Rizza, M.; Instituto Nacional de Investigación Agropecuaria (INIA), Unidad de...Biblioteca(s): INIA Las Brujas. |
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12. | | MAIDANA, M.; MURCHIO, S.; LEONI, C.; SEÑORALE, M.; DALLA RIZZA, M. Plataformas de expresión funcional del péptido antimicrobiano Aq-AMP2 y su potencial aplicación en patosistemas vegetales. In: JORNADA NACIONAL DE FITOPATOLOGÍA, 3; JORNADA NACIONAL DE PROTECCIÓN VEGETAL, 1., 3 SETIEMBRE 2015, MONTEVIDEO, URUGUAY. Libro de Resúmenes. Montevideo (Uruguay) : SUFIT, 2015. p. 40 Financiamiento: Beca de Maestría ANII, INIA Proyecto BT_11 ?Biotecnología de péptidos antimicrobianos.Biblioteca(s): INIA Las Brujas. |
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14. | | OBERTI, H.; REYNO, R.; MURCHIO, S.; GOÑI, C.; DALLA RIZZA, M. Primer borrador del genoma de Paspalum malacophyllum, especie de relevancia como fuente de inmunidad al ergot en el pasto P. dilatatum. [resumen]. In: REDBIO México 2022, XI Congreso, "Biotecnología productiva y sostenible". Libro de resúmenes. 12-14 octubre 2022, Yucatán, México. p.20 Este proyecto fue financiado por INIA proyecto BT-19.Biblioteca(s): INIA Las Brujas. |
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16. | | ESTEVES, P.; MURCHIO, S.; CEPPA, M.; BONILLA, B.; DIEPPA, D.; CASTILLO, A.; DALLA RIZZA, M. Puesta en marcha del Proyecto: Producción de Haploides-duplicados de arroz, trigo y cebada. In: INIA (Instituto Nacional de Investigación Agropecuaria); Inia Las Brujas; Biotecnología. Apostando a la investigación para un futuro innovador. Jornada de Agrobiotecnología, IX. Jornada Técnica. Canelones (Uruguay): INIA , 2015. p. 17-19 (Actividades de Difusión; 755)Biblioteca(s): INIA Las Brujas. |
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20. | | BOSCHI, F.; VILARÓ, F.; GALVÁN, G.; SIRI, M. I.; FERREIRA, V.; MURCHIO, S.; SCHVARTZMAN, C.; DALLA RIZZA, M. Aspectos de bioseguridad en la evaluación de papa modificada genéticamente para el control de Ralstonia solanacearum. [p19]. Bloque 2: Mejoramiento por resistencia a enfermedades. In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 4., Jornada Uruguaya de Protección Vegetal, 2., 1° setiembre, 2017, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2017. p. 39.Biblioteca(s): INIA Las Brujas. |
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Registros recuperados : 34 | |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
05/04/2022 |
Actualizado : |
01/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
SCHVARTZMAN, C.; FRESIA, P.; MURCHIO, S.; MUJICA, V.; DALLA RIZZA, M. |
Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
RNAi in Piezodorus guildinii (Hemiptera: Pentatomidae): transcriptome assembly for the development of pest control strategies. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Frontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839 |
Páginas : |
13 p. |
DOI : |
10.3389/fpls.2022.804839 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022.
Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy
Al abrir el registro se visualiza el enlace al material suplementario. |
Contenido : |
Red-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. |
Palabras claves : |
DSRNA,; PEST CONTROL; RNA-SEQ; RNAI; STINK BUG; VATPASE A,. |
Asunto categoría : |
H10 Plagas de las plantas |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16649/1/Frontiers-Plant-Science-2022-Schvartzman.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/16650/1/Frontiers-Plant-Science-2022-Schvartzman-1.PDF
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Marc : |
LEADER 02536naa a2200277 a 4500 001 1062960 005 2022-09-01 008 2022 bl uuuu u00u1 u #d 024 7 $a10.3389/fpls.2022.804839$2DOI 100 1 $aSCHVARTZMAN, C. 245 $aRNAi in Piezodorus guildinii (Hemiptera$bPentatomidae): transcriptome assembly for the development of pest control strategies.$h[electronic resource] 260 $c2022 300 $a13 p. 500 $aArticle history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022. Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy Al abrir el registro se visualiza el enlace al material suplementario. 520 $aRed-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. 653 $aDSRNA, 653 $aPEST CONTROL 653 $aRNA-SEQ 653 $aRNAI 653 $aSTINK BUG 653 $aVATPASE A, 700 1 $aFRESIA, P. 700 1 $aMURCHIO, S. 700 1 $aMUJICA, V. 700 1 $aDALLA RIZZA, M. 773 $tFrontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839
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